Caprisil C18-AQ Prep Column

Caprisil C18-AQ is a high density C18 packing specifically engineered for the retention of polar analytes. Caprisil C18-AQ is a reversed phase column that enables highly soluble solutes to fully interact with the bonded hydrocarbon phase, even in pure aqueous mobile phases. Under these highly aqueous conditions, our novel proprietary bonding chemistry allows the bonded chains to remain fully extended in the mobile phase. Caprisil C18-AQ can retain highly water-soluble compounds such as small organic acids, water-soluble vitamins, purines and pyrimidines, cathecholamines and other polar compounds.

Caprisil C18-AQ is a water-based column resistant to 100% water phase, suitable for high water mobile phase analysis, can provide an option for the analysis of larger polar compounds. There are hydrophilic groups on the surface of the chromatographic packing, which increase the interaction. When separating polar compounds in high aqueous phase system, no change in retention characteristics will occur, and the theoretical plate number and chromatographic peak separation performance will be greatly improved.

Mobile phase: A acetonitrile   B 0.1% phosphoric acid water

Gradient:0 min=5%A    20 min=100 %A

Flow rate: 1 ml/min

Detector: UV 280nm

Injection volume: 10ul

Sample: 1.protocatechuic acid 2.chlorogenic acid 3.ferulic acid 4.coumarin

Column: Caprisil C18-AQ 250×4.6mm, 5μm

Mobile phase: 50mM H3PO4

Flow rate: 1 ml/min

Detector: UV 210nm

Sampling volume: 5 ul

Sample: 1.Lactic 2.citric acid 3.fumaric acid 4.gallic acid